Inhibition:Article Title: Nicotine engages a VTA-NAc feedback loop to inhibit amygdala-projecting dopamine neurons and induce anxiety
Article Snippet: .. To perform non-conditional optogenetic inhibition of the NAc inputs onto the VTA for tetrode recordings, we injected pAAV-hsyn-Jaws-KGC-GFP-ER2 (1,3e 1:10 dilution; 65014; Addgene) or ssAAV5/2-hSyn1-JAWS-KGC-EGFP-ER2-WPRE-hGHp(A) (5,9e ; v387-5; VVF Zurich, from #65014 of Addgene) in the 3 subregions of the NAc (200 nl each site: NAcMSh: bregma +1.78 mm, lateral 0.45 mm, ventral 4.1 mm; NAcCore: bregma +1.54 mm, lateral 1.0 mm, ventral 4.0 mm; NAcLSh: bregma +1.45 mm, lateral 1.75 mm, ventral 4.0 mm) in WT mice. .. To perform optogenetic behavioral experiments (EOM test, Open field), we did bilateral injections of ssAAV5/2-hSyn1-JAWS-KGC-EGFP-ER2-WPRE-hGHp(A) (5,9e ; v387-5; VVF Zurich, from #65014 of Addgene) or ssAAV5/2-hSyn1-EGFP-WPRE-hGHp(A) (5,7e ; v81-5; VVF Zurich) in the NAc (200 nl each site: NAcMSh: bregma +1.78 mm, lateral 0.45 mm, ventral 4.1 mm; NAcCore: bregma +1.54 mm, lateral 1.0 mm, ventral 4.0 mm; NAcLSh: bregma +1.45 mm, lateral 1.75 mm, ventral 4.0 mm) in WT mice.
Article Title: Nicotine engages a VTA-NAc feedback loop to inhibit amygdala-projecting dopamine neurons and induce anxiety-like behaviors.
Article Snippet: .. To perform non-conditional optogenetic inhibition of the NAc inputs onto the VTA for tetrode recordings, we injected pAAV-hsynJaws-KGC-GFP-ER2 (1,3e13 1:10 dilution; 65014; Addgene) or ssAAV5/2hSyn1-JAWS-KGC-EGFP-ER2-WPRE-hGHp(A) (5,9e12; v387-5; VVF Zurich, from #65014 of Addgene) in the 3 subregions of the NAc (200nl each site: NAcMSh: bregma +1.78mm, lateral 0.45mm, ventral 4.1mm; NAcCore: bregma +1.54mm, lateral 1.0mm, ventral 4.0mm; NAcLSh: bregma +1.45mm, lateral 1.75mm, ventral 4.0mm) in WT mice. .. To perform optogenetic behavioral experiments (EOM test, Open field), we did bilateral injections of ssAAV5/2-hSyn1-JAWS-KGC-EGFPER2-WPRE-hGHp(A) (5,9e12; v387-5; VVF Zurich, from #65014 of Addgene) or ssAAV5/2-hSyn1-EGFP- WPRE-hGHp(A) (5,7e12; v81-5; VVF Zurich) in theNAc (200nl each site:NAcMSh: bregma+1.78mm, lateral 0.45mm, ventral 4.1mm; NAcCore: bregma +1.54mm, lateral 1.0mm, ventral 4.0mm; NAcLSh: bregma +1.45mm, lateral 1.75mm, ventral 4.0mm) in WT mice.
Article Title: Nicotine engages a VTA-NAc feedback loop to inhibit amygdala-projecting dopamine neurons and induce anxiety-like behaviors
Article Snippet: .. To perform non-conditional optogenetic inhibition of the NAc inputs onto the VTA for tetrode recordings, we injected pAAV-hsyn-Jaws-KGC-GFP-ER2 (1,3e 13 1:10 dilution; 65014; Addgene) or ssAAV5/2-hSyn1-JAWS-KGC-EGFP-ER2-WPRE-hGHp(A) (5,9e 12 ; v387-5; VVF Zurich, from #65014 of Addgene) in the 3 subregions of the NAc (200 nl each site: NAcMSh: bregma +1.78 mm, lateral 0.45 mm, ventral 4.1 mm; NAcCore: bregma +1.54 mm, lateral 1.0 mm, ventral 4.0 mm; NAcLSh: bregma +1.45 mm, lateral 1.75 mm, ventral 4.0 mm) in WT mice. .. To perform optogenetic behavioral experiments (EOM test, Open field), we did bilateral injections of ssAAV5/2-hSyn1-JAWS-KGC-EGFP-ER2-WPRE-hGHp(A) (5,9e 12 ; v387-5; VVF Zurich, from #65014 of Addgene) or ssAAV5/2-hSyn1-EGFP- WPRE-hGHp(A) (5,7e 12 ; v81-5; VVF Zurich) in the NAc (200 nl each site: NAcMSh: bregma +1.78 mm, lateral 0.45 mm, ventral 4.1 mm; NAcCore: bregma +1.54 mm, lateral 1.0 mm, ventral 4.0 mm; NAcLSh: bregma +1.45 mm, lateral 1.75 mm, ventral 4.0 mm) in WT mice.
Injection:Article Title: Nicotine engages a VTA-NAc feedback loop to inhibit amygdala-projecting dopamine neurons and induce anxiety
Article Snippet: .. To perform non-conditional optogenetic inhibition of the NAc inputs onto the VTA for tetrode recordings, we injected pAAV-hsyn-Jaws-KGC-GFP-ER2 (1,3e 1:10 dilution; 65014; Addgene) or ssAAV5/2-hSyn1-JAWS-KGC-EGFP-ER2-WPRE-hGHp(A) (5,9e ; v387-5; VVF Zurich, from #65014 of Addgene) in the 3 subregions of the NAc (200 nl each site: NAcMSh: bregma +1.78 mm, lateral 0.45 mm, ventral 4.1 mm; NAcCore: bregma +1.54 mm, lateral 1.0 mm, ventral 4.0 mm; NAcLSh: bregma +1.45 mm, lateral 1.75 mm, ventral 4.0 mm) in WT mice. .. To perform optogenetic behavioral experiments (EOM test, Open field), we did bilateral injections of ssAAV5/2-hSyn1-JAWS-KGC-EGFP-ER2-WPRE-hGHp(A) (5,9e ; v387-5; VVF Zurich, from #65014 of Addgene) or ssAAV5/2-hSyn1-EGFP-WPRE-hGHp(A) (5,7e ; v81-5; VVF Zurich) in the NAc (200 nl each site: NAcMSh: bregma +1.78 mm, lateral 0.45 mm, ventral 4.1 mm; NAcCore: bregma +1.54 mm, lateral 1.0 mm, ventral 4.0 mm; NAcLSh: bregma +1.45 mm, lateral 1.75 mm, ventral 4.0 mm) in WT mice.
Article Title: Nicotine engages a VTA-NAc feedback loop to inhibit amygdala-projecting dopamine neurons and induce anxiety-like behaviors.
Article Snippet: .. To perform non-conditional optogenetic inhibition of the NAc inputs onto the VTA for tetrode recordings, we injected pAAV-hsynJaws-KGC-GFP-ER2 (1,3e13 1:10 dilution; 65014; Addgene) or ssAAV5/2hSyn1-JAWS-KGC-EGFP-ER2-WPRE-hGHp(A) (5,9e12; v387-5; VVF Zurich, from #65014 of Addgene) in the 3 subregions of the NAc (200nl each site: NAcMSh: bregma +1.78mm, lateral 0.45mm, ventral 4.1mm; NAcCore: bregma +1.54mm, lateral 1.0mm, ventral 4.0mm; NAcLSh: bregma +1.45mm, lateral 1.75mm, ventral 4.0mm) in WT mice. .. To perform optogenetic behavioral experiments (EOM test, Open field), we did bilateral injections of ssAAV5/2-hSyn1-JAWS-KGC-EGFPER2-WPRE-hGHp(A) (5,9e12; v387-5; VVF Zurich, from #65014 of Addgene) or ssAAV5/2-hSyn1-EGFP- WPRE-hGHp(A) (5,7e12; v81-5; VVF Zurich) in theNAc (200nl each site:NAcMSh: bregma+1.78mm, lateral 0.45mm, ventral 4.1mm; NAcCore: bregma +1.54mm, lateral 1.0mm, ventral 4.0mm; NAcLSh: bregma +1.45mm, lateral 1.75mm, ventral 4.0mm) in WT mice.
Article Title: Nicotine engages a VTA-NAc feedback loop to inhibit amygdala-projecting dopamine neurons and induce anxiety-like behaviors
Article Snippet: .. To perform non-conditional optogenetic inhibition of the NAc inputs onto the VTA for tetrode recordings, we injected pAAV-hsyn-Jaws-KGC-GFP-ER2 (1,3e 13 1:10 dilution; 65014; Addgene) or ssAAV5/2-hSyn1-JAWS-KGC-EGFP-ER2-WPRE-hGHp(A) (5,9e 12 ; v387-5; VVF Zurich, from #65014 of Addgene) in the 3 subregions of the NAc (200 nl each site: NAcMSh: bregma +1.78 mm, lateral 0.45 mm, ventral 4.1 mm; NAcCore: bregma +1.54 mm, lateral 1.0 mm, ventral 4.0 mm; NAcLSh: bregma +1.45 mm, lateral 1.75 mm, ventral 4.0 mm) in WT mice. .. To perform optogenetic behavioral experiments (EOM test, Open field), we did bilateral injections of ssAAV5/2-hSyn1-JAWS-KGC-EGFP-ER2-WPRE-hGHp(A) (5,9e 12 ; v387-5; VVF Zurich, from #65014 of Addgene) or ssAAV5/2-hSyn1-EGFP- WPRE-hGHp(A) (5,7e 12 ; v81-5; VVF Zurich) in the NAc (200 nl each site: NAcMSh: bregma +1.78 mm, lateral 0.45 mm, ventral 4.1 mm; NAcCore: bregma +1.54 mm, lateral 1.0 mm, ventral 4.0 mm; NAcLSh: bregma +1.45 mm, lateral 1.75 mm, ventral 4.0 mm) in WT mice.
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